Practical: Electrophoresis of Serum Proteins - Supplementum
 
Medical Chemistry & Biochemistry 1st year / Pathobiochemistry 3rd year


Serum proteins
70 g/l
albumin
40 g/l
(60 % ser. prot.)
    Essential for oncotic pressure of plasma. Transport of elements, hormones, bilirubin, drugs etc. Major protein pool for metabolism. Produced in liver.
catabolism
hepatopathy
protein loss
α1-globulins
3 g/l
(4 % ser. prot.)
α1-antitrypsin (AAT)
1-protease inhibitor, API)
2 g/l
(60 % of α1-glob.)
Potent inhibitor of proteolytic enzymes that are released by macrophages in inflammation (elastase, collagenase). Produced in liver.
acute inflammation
hepatopathy
α1-lipoprotein (α1-Lp)
1 g/l
(30 % of α1-glob.)
High-density lipoprotein (HDL). Transport of cholesterol and lipids. Produced in liver.  
α2-globulins
6 g/l
(8 % ser. prot.)
haptoglobin (Hp)
2 g/l
(30 % of α2-glob.)
Scavenger of free haemoglobin. Produced in liver.
acute inflammation
hepatopathy
intravascular haemolysis (consumption of haptoglobin)
α2-macroglobulin (α2-M) 3 g/l
(50 % of α2-glob.)
Inhibitor of proteases (trypsin, chymotrypsin, plasmin). High molecular weight of this protein (820,000 g/mol) prevents it to leak even through damaged glomerular membrane. Produced in liver.
acute inflammation
ß-globulins
9 g/l
(12 % ser. prot.)
several components of complement
(C1r, C2, C3, C4, C5)
together 2 g/l
(20 % of ß-glob.)
Proteins involved in both specific and non-specific immune response, their activation leads to lysis of bacteria or cells.  
ß-lipoprotein (ß-Lp)
1 g/l
(10 % of ß-glob.)
Low-density lipoprotein (LDL). Transport of cholesterol and lipids. High molecular weight. Produced in liver.  
transferrin (Tf)
3 g/l
(30 % of ß-glob.)
Transporter and scavenger of iron. Produced in liver.
lack of iron
hepatopathy
iron overload
acute state
γ-globulins
12 g/l
(16 % ser. prot.)
immunoglobulin G (IgG)
10 g/l
(80 % of γ-glob.)
Major antibodies prevalent especially in chronic inflammation.
chronic inflammation
immunoglobulin M (IgM)
1 g/l
(10 % of γ-glob.)
Early antibodies.
inflammation
immunoglobulin A (IgA)
1 g/l
(10 % of γ-glob.)
Antibodies related
to mucous (GIT, respiratory tract, conjunctiva etc.)
inflammation
© MUDr. Martin Vejražka

 
 
  .:: created by Lukáš Mikšík, lukas@miksik.com :: edited by Jan Pláteník, jplat@lf1.cuni.cz